Journal of Applied Animal Research
  • Year: 2008
  • Volume: 33
  • Issue: 2

A New Method of Brain Staining with Triphenyltetrazolium Chloride to Determine the Infarct Size in Rats

  • Author:
  • E. Kheradpezhouh1,2,, R. Miri2, A. Noorafshan3, M.R. Panjehshahin1, D. Mehrabani4
  • Total Page Count: 4
  • Page Number: 149 to 152

1Department of Pharmacoloy, Shiraz Medical School, Shiraz University of Medical Sciences, Shiraz, 71955-848, Iran

2Medicinal and Natural Products Chemistry Research Center, Shiraz Medical School, Shiraz University of Medical Sciences, Shiraz, 71955-848, Iran

3Department of Anatomy, Shiraz Medical School, Shiraz University of Medical Sciences, Shiraz, 71955-848, Iran

4Gastroenterohepatology Research Center, Nemazee Hospital, Shiraz Medical School, Shiraz University of Medical Sciences, Shiraz, 71955-848, Iran

*For correspondence: Tel: +98-711-230-7591; Fax: +98-711-230-7594; E-mail: ekherad@sums.ac.ir

Abstract

To show the accuracy of 2, 3, 5-Triphenyltetrazolium chloride (TTC) staining method, in a case-control study, 10 rats were divided into two groups, an ischemia-induced (experimental) and a control group. The brains of both groups were stained with the new method. The complete staining of the brain of all rats in the control group confirmed the efficacy of this method. Besides, in the experimental group, the method distinguished between the viable and dead tissues and could demonstrate the borders and the size of the infarction too.

Keywords

2, 3, 5-triphenyltetrazolium chloride (TTC), cerebral ischemia, stereology