Journal of Applied Animal Research
  • Year: 2008
  • Volume: 33
  • Issue: 2

Molecular Variability of Somatotropin Releasing Hormone (SRH) Gene in Mithun (Bos frontalis)+

  • Author:
  • Subodh Kumar, V. Ram Prasath Manohar1, S.M. Deb2, Abhijit Mitra, Bejoy John, Arjava Sharma, K.M. Bujarbaruah3
  • Total Page Count: 3
  • Page Number: 205 to 207

Animal Genetics Division, Indian Veterinary Research Institute, Izatnagar-243 122, India

*Corresponding author: Tel: +91-581-2303382, Fax: +91-581-2303284; E-mail: subodh@ivri.up.nic.in, subkum@gmail.com

Present address: 1Veterinary Asstt. Surgeon, Chennai, Tamil Nadu

Abstract

A fragment of 452 bp corresponding to exon 2 and 3 of somatotropin releasing hormone (SRH) gene on amplification in 90 mithun (Bos frontalis) revealed two genotypic patterns viz. AA genotypic pattern (bands of 292 and 160 bp) and AB genotypic pattern (452, 292, and 160 bp) with respect to SauI restriction enzyme. The sequence of the A allele, which was the first report on mithun SRH gene, was submitted to GenBank (Accession No. EF014289).

Keywords

Bos frontalis, exon 2–3, mithun, PCR-RFLP, SauI, somatotropin releasing hormone