Journal of Applied Animal Research

Sheep buccal glands consist of mucous acini capped by demilunes. Information on the chemical structure of their secretory glycoconjugates were obtained by means of a battery of peroxidase conjugated lectins with affinity for specific terminal and/or internal sugars. Neuraminidase procedures followed by lectin staining were also used to visualize the carbohydrate sequence. Stored secretions in mucous acinar cells contained glycoconjugates with N-acetylglucosamine and sialic acid linked to α,N-acetylgalactosamine and galactosyl (β1→3) N-acetylgalactosamine. Demilunar cells displayed fucose, mannose, N-acetylglucosamine and α,N-acetylgalactosamine residues. Cells lining duct system showed a very strong staining at the apical surface and in the cytoplasm with UEA I, LTA and ConA.