Journal of Applied Animal Research

  • Year: 2009
  • Volume: 35
  • Issue: 1

Reliable and Fast Molecular Sexing in Bos indicus Cattle Using Amelogenin Gene Primers

  • Author:
  • K.J. Khaledi1,5,, J.M. Panandam1,2, A.A. Maheran3, S.S. Siraj4
  • Total Page Count: 3
  • DOI:
  • Page Number: 83 to 85

1Department of Animal Science, Faculty of Agriculture, Universiti Putra Malaysia, 43400, UPM Serdang, Malaysia.

2Institute of Tropical Agriculture, Universiti Putra Malaysia, 43400, UPM Serdang, Malaysia.

3Department of Agriculture Technology, Faculty of Agriculture, Universiti Putra Malaysia, 43400, UPM Serdang, Malaysia.

4Department of Biology, Faculty of Science, Universiti Putra Malaysia, 43400, UPM Serdang, Malaysia.

5Department of Agriculture, Islamic Azad University Shahr-e-Ray, Tehran, Iran.

*Corresponding author: E-mail: k_khaledi2000@yahoo.com

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Abstract

To investigate reliability of amelogenin gene primer pair for exon 5, SE47/SE48 for sex differentiation in Bos indicus three male and three female Brakmas (B. indicus) cattle were tested. The SE 47/48 primer pair generated PCR products of fragment length 280 bp for the females (AMELX) and fragments of 280 bp (AMELX) and 213 bp (AMELY) for the males. Thus the SE47/48 primer pair is not only reliable for sex differentiation in B. indicus but may also be used to differentiate the B. indicus and B. taurus males.

Keywords

Bos indicus, amelogenin, sex differentiation