Animal Genetics Division, Indian Veterinary Research Institute, Izatnagar-243 122, India.
*Corresponding author: Tel: +91-581-2303382, Fax: +91-581-2303284; E-mail: subkum@gmail.com, subodh@ivri.up.nic.in
Present address: 1Veterinary Asstt. Surgeon, Chennai, Tamilnadu.
A fragment of 544 bp corresponding to exon 1 and 2 of somatotropin hormone (STH) gene was amplified in 90 mithun (Bos frontalis). The nucleotide sequence similarity of mithun with zebu cattle, yak, exotic cattle and buffalo was found to be 99.6, 99.4, 98.9 and 93.5%, whereas, the corresponding figures for deduced amino acid sequence were 100, 100, 99.4 and 98.8%, respectively. The PstI and PvuII PCR-RFLP analysis produced bands of 479, 65 and 448, 96 bp, respectively, in all the animals indicating no mutant allele and hence monomorphism in the mithun population studied. The sequence of the amplicon was submitted to GenBank (Accn no. EU166347).
Bos frontalis, exon 1–2, mithun, PCR-RFLP, Pst I, PvuII, somatotropin hormone
