1College of Animal Science and Technology, Yangzhou University, Yangzhou, 225009, China.
2Faculty of Veterinary Science, University of Nyala, P.O. Box 155, Nyala, Sudan.
*Corresponding author: E-mail: wenbinbao74@yahoo.com
For sex identification nondestructive sampling method was used to collect feathers from Siberian White crane to extract genomic DNA. Thereafter, three combinations of primers were used to amplify the EE0.6 sequence. The male birds displayed only one distinct amplified band, whereas, females displayed two bands. The results concluded that PCR technique for sex-related gene amplification is successful for sex identification of Siberian White crane. This method may also guide for accurate sex identification of other endangered birds.
EE0.6 sequence, Siberian White crane, sex identification
