Journal of Applied Animal Research
  • Year: 2009
  • Volume: 35
  • Issue: 2

Evaluation of PCR and IFAT in the Diagnosis of Canine Ehrlichiosis

  • Author:
  • Asha Rajagopal1,, S. Abdul Basith2, S. Gomathinayagam2, G. Dhinakarraj3
  • Total Page Count: 3
  • Page Number: 189 to 191

1Department of Veterinary Parasitology, College of Veterinary & Animal Sciences, Mannuthy, Thrissur, Kerala, India.

2Department of Veterinary Parasitology, Madras Veterinary College, Chennai-600 007, India.

3Department of Animal Biotechnology, Madras Veterinary College, Chennai-600 007, India.

*E-mail: ashasrj@yahoo.co.in

Abstract

The results of nested PCR were correlated with that of indirect fluorescent antibody test (IFAT) on 50 matched samples. The results were concordant in 44 out of 50 cases. PCR was concluded to be more suitable than IFAT in the diagnosis of early and subclinical infections of canine ehrlichiosis and served as a reliable indicator for determining the significance of persistent antibody titers.

Keywords

Ehrlichia canis, nested PCR, IFAT