Histomorphological and Angiogenesis Observations in the Heart of the Male Puppy Following Ligation of the Paraconal Artery

To study the effect of ligation of paraconal artery, simulating a coronary blockage, six 2-month-old male puppies were divided into three groups. Two puppies each were used as a control group, operated for histomorphological investigations (group A) and used for angiogenesis observations (group B). In operated animals (groups A and B) paraconal trunks were ligated at the base. Animals were euthenized after 45 d and their hearts were dissected out. In group A, 3 parts on the wall of the left ventricle, according to the vicinity to the ligated vessel, were separated as I, II, III parts, fixed and studied by light and electron microscope. In group B contrast medium was injected into the coronary sinuses above the aortic valves and radiographs were taken. No ischemic changes were seen in the chosen parts of the heart due to the growth and development of circumflex branches and anastomotic process, which supplied the heart wall.