Molecular Genetics Laboratory, Central Avian Research Institute, Izatnagar-243 122, India.
** Corresponding author E-mail: skgcari@yahoo.co.uk
1Present addresses: Aquatic Biotechnology & Fish Pathology, Laboratory, Department of Animal Science, M.J.P Rohilkhand, University, Bareilly India.
DNA polymorphism at Interleukin-2R γ-chain (IL-2Rγ) and Interleukin-15R α-chain (IL-15Rα) genes were studied by PCR-RFLP technique in 48 random bred Kadaknath native chicken, 24 in high and 24 in low immunocompetence (IC) index groups. Amplification of these regions yielded products of 600 and 1120 bp, respectively. Hph I digestion of IL-2Rγ PCR product generated fragments of 465 and 454 bp sizes. Two types of homozygotes (465bp and 454 bp fragments) and heterozygotes (both 465 and 454 bp fragments) were observed. The gene and genotypic frequencies of A and B alleles and AA, BB and AB genotypes were 0.19 and 0.81 and 2.33, 65.12 and 32.56, respectively. PCR-RFLP analysis of IL-2Rγ gene with Pst 1, Dde 1 and Taq 1 showed monomorphic pattern in all the individuals. High IC-index group demonstrated higher magnitude of all IC-traits. BB genotype demonstrated higher HA titre than the AB genotype in both the lines. Hinf I PCR-RFLP of IL-15Rα revealed monomorphic banding pattern in all the samples analyzed. Hph I PCR-RFLP of IL-2Rγ region may lead to development of molecular marker for high humoral immune response in chicken.
Kadakanath, native chicken, polymorphism, IL-2Rγ, IL-15Rα, PCR-RFLP
