1Lanzhou Institute of Animal Science & Veterinary Pharmaceutics Chinese Academy of Agricultural Sciences Lanzhou, 730050, P.R. China.
2Gansu Agricultural University, Lanzhou, 730030, P.R. China.
*For correspondence: 335, Jiangouyan, Qilihe district, Lanzhou city 730050, P.R. China; E-mail: guoxian2006@sina.com.
To study the effect of sheep twinning after tandem inhibin gene immunization, the recombinant plasmid of tandem inhibin were constructed with inhibin α-subunit (1–32) of pig and complement 3d of sheep by RT-PCR, which were used to immunize the sheep. The recombinant plasmid, pcDNA-DPPISS-DINH and pcDNA-DPPISS-DINH-sC3d3 were constructed successfully. After BHK-21 cells were transfected with the recombinant plasmid pcDNA-DPPISS-DINH and pcDNA-DPPISS-DINH-sC3d3, secreted expression was obtained. After immunization, the twinning rate of sheep was 12.5% and 25.0%, respectively, which was significantly higher (P<0.05) than the control group. The construction of recombinant plasmid of tandem inhibin gene made the theoretical and technical basis for developing the inhibin gene as vaccine for sheep.
Inhibin, gene immunization, twinning rate, sheep
