Journal of Applied Animal Research
  • Year: 2010
  • Volume: 37
  • Issue: 2

β-Tubulin Gene Based PCR-RFLP Method for Specific Detection of Babesia bigemina and Theileria annulata Isolates

  • Author:
  • Harkirat Singh1,†, P.S. Cheema2, A.K. Mishra1,, A.K. Tewari1, J.R. Rao1, R. Ravindran3, D. Ray1, G.C. Bansal1
  • Total Page Count: 6
  • Page Number: 233 to 238

1Division of Parasitology, Indian Veterinary Research Institute, Izatnagar 243 122, India.

2Division of Bacteriology and Mycology, Indian Veterinary Research Institute, Izatnagar 243 122, India.

3Department of Parasitology, College of Veterinary and Animal Sciences, Pookot 673 576, India.

* Corresponding author e-mail: drashokkumarmishra@gmail.com

†Present address: Asstt. Prof., Deptt. of Vety. Parasitology, College of Vety. Science, GADVASU, Ludhiana 141 004.

Abstract

For early detection of Babesia bigemina and Theileria annulata β-tubulin gene was targeted based on PCR assays. Subsequently, on the basis of sequence analysis of β-tubulin gene fragment of various isolates, a new PCR-RFLP method based on enzymes EcoRI and HindIII has been developed for species identification of B. bigemina and T. annulata isolates.

Keywords

Babesia bigemina, β-tubulin, cattle, PCR-RFLP, Theileria annulata