Journal of Applied Animal Research

  • Year: 2010
  • Volume: 37
  • Issue: 2

Molecular Characterisation of Indian Strains of Mycobacteriumavium subspecies paratuberculosis by Pulsed-Field Gel Electrophoresis

  • Author:
  • B.N. Tripathi1,, K. Stevenson2
  • Total Page Count: 5
  • DOI:
  • Page Number: 247 to 251

1Division of Pathology, Indian Veterinary Research Institute, Izatnagar-243 122, India.

2Division of Bacteriology, Moredun Research Institute, International Research Centre Pentlands Science Park Penicuik, Midlothian, Scotland, UK.

* For Correspondence: E-mail: bntripathi1@yahoo.co.in.

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Abstract

Indian isolates of Mycobacterium avium subspecies paratuberculosis (MAP) from cattle (5) and goats (4) were characterised by pulsed-field gel electrophoresis (PFGE) and polymerase chain reaction (PCR) and profiles were compared with standard strains. All isolates were found to have IS 900 and F57 genes. PFGE analysis with SnaBI revealed that the profiles of the cattle and goat isolates were identical and had a unique profile when compared with profiles currently in the PFGE database. PFGE analysis with SpeI demonstrated that all of the isolates were identical to SpeI profile 1 in the database. It was concluded that Indian isolates from cattle and goats were genetically similar but different from European strains.

Keywords

Indian strains, molecular characterization, Mycobacterium avium subspecies paratuberculosis, PFGE