Cotton waste as an optimal substrate for cultivation of the pink oyster mushroom Pleurotus djamor

Pleurotus djamor, commonly known as the pink oyster mushroom, has garnered attention for its significant medicinal and nutritional value. Due to the limited scientific information available on the optimal cultivation conditions for this macro fungus, the objective of this study was to identify suitable temperature, media, and spawning material for upscaling mycelium, as well as an appropriate substrate for cultivating the P. djamor strain PN2. The mycelium of strain PN2 exhibited the most robust growth when cultured on YMA and Raper media at 25°C. The optimal spawning material for upscaling the mycelium of strain PN2 comprised 79% rice grain, 18% sawdust, 2% rice bran, and 1% CaCO. Additionally, cotton waste was identified as the optimal substrate for cultivating P. djamor, resulting in a remarkable biological efficiency of 48.56%. Collectively, our findings provide useful information for the industrialization of P. djamor cultivation.