Sensitive and cost-effective citrate-based RNA extraction procedure for isolation of RNA from Tilapia Lake Virus-infected fish

Aquaculture plays a crucial role in improving global nutrition and food security, with tilapia being a widely farmed fish species. However, the emergence of Tilapia Lake Virus Disease (TiLVD) poses a significant threat to the aquaculture industry and food security. This study aims to develop a novel and cost-effective RNA extraction method for the accurate diagnosis of TiLVD. It utilizes a Citrate buffer-based approach, which eliminates the need for harsh organic solvents and DNase treatment, simplifying the extraction process, reducing costs, and promoting environmental sustainability. The conventional Trizol method was used as a control for comparison. The results of the study demonstrate the efficiency and effectiveness of the Citrate buffer-based RNA extraction method in comparison to the Trizol technique. This novel method has the potential to enhance disease surveillance, facilitate early detection, and enable effective management strategies for TiLVD. Furthermore, high-quality RNA samples obtained through this method may contribute to a better understanding of TiLVD pathogenesis, viral load dynamics, and host–virus interactions. Overall, the development of a robust and affordable RNA extraction method specifically tailored for TiLVD diagnosis holds immense potential for the aquaculture industry. It addresses the limitations of existing techniques and contributes to the sustainable management of tilapia aquaculture.