Guided Tissue Regeneration with β-Tricalcium Phosphate and Platelet-rich Plasma for Fracture Repair in Dogs using Internal Fixation

The study was conducted on 19 adult dogs referred for treatment of fracture in diaphyseal region of three long bones, viz, radius-ulna, femur and tibia-fibula. These dogs were divided randomly into three groups viz T1, T2 and T3. In group T1, the diaphyseal fractures of the long bones were immobilized by an internal fixation technique using reconstruction plate or locking compression plate. In group T2, β-tri calcium phosphate granules were incorporated at fracture site after fixation. In group T3, one milliliter of PRP was administered locally at fracture site just before the closing the first suture line. Clinical and radiographic examination were also conducted during the study. There was a significant reduction in mean inflammation score at 7^th day in groups T2 and T3 and at 30^th day than 0 day in all the groups. At 90^th day, the mean radiographic score was highest for group T3 and it was followed by the score in group T2. At day 90^th, a radiograph in both the views was not able to delineate the granules from osseous tissue. The mean weight bearing score was also higher in group T3 than in group T2 at 30^th, 60^th and 90^th post operative day showing that restoration of the function was better and earlier in groups T2 and T3 than in group T1.