1College of Biotechnology, DUVASU, Mathura, India
2Department of Animal Genetics and Breeding, C.o.V.Sc. & A.H., DUVASU, Mathura, India
3Department of Veterinary Biochemistry, C.o.V.Sc. & A.H., DUVASU, Mathura, India
4Department of Animal Husbandry and Extension, C.o.V.Sc. & A.H., DUVASU, Mathura, India
*Corresponding author: SP Singh; E-mail: satsinpal21@gmail.com
Online published on 17 October, 2020.
Booroola (FecB) gene is also known as Bone morphogenetic protein receptor type 1B (BMPR1B) gene, expressed in oocytes and granulosa cells. BMPR1B gene has been characterized by a point mutation at 746th position (A to G) leading to a non-synonymous substitution of Glutamine with an Arginine at 249th position (Q249R) produced ‘hyperprolific’ Booroola sheep. This mutation was associated with prolificacy in sheep with increase ovulation rate. In the present study, DNA was isolated from blood samples collected from the Muzzafarnagari sheep breeds (n = 200) maintained at LFC (Livestock farm complex) of DUVASU, Mathura, U.P. The AvaII/PCR-RFLP assay of 190 bp amplified product of FecB gene revealed only ++ genotype (190 bp uncut, 100%) with + allele (1.0) in screened sheep population. The studied region of the FecB gene showed monomorphic pattern revealed that FecB A (wild type; +) allele seems to be fixed in screened sheep population. Consequently, we could not perform association analysis with reproduction trait. Therefore, it would be suggested to further screening the status of this SNP along with other fecundity genes in large diversified population to exploit it under marker assisted selection.
HIGHLIGHTS
• The AvaII/PCR-RFLP assay of 190 bp FecB amplified product revealed only AA (++) genotype (100%) with A (+) allele (1.0).
• FecB gene showed monomorphic pattern revealed that FecB A (wild type; +) allele seems to be fixed in screened sheep population.
Booroola, FecB, BMPR1B, PCR- RFLP, Muzzafarnagari
