1Department of Veterinary Microbiology, College of Veterinary Science & A.H., SDAU, Sardarkrushinagar, Gujarat, India
2Department of Veterinary Pathology, College of Veterinary Science & A.H., SDAU, Sardarkrushinagar, Gujarat, India
3Department of Animal Biotechnology, College of Veterinary Science & A.H., SDAU, Sardarkrushinagar, Gujarat, India
*Corresponding author: KK Sharma; E-mail: kishan12sharma@rediffmail.com
Online published on 27 May, 2021.
In the present study a lung sample was collected from autopsied rabbit, which was suspected to have died due to E. coli infection and cultured on MacConkey agar. Itrevealed typical cultural and biochemical characteristics of E. coli. Molecular confirmation of E. coli was carried out using 16s RNA (ECO1) universal eubacterial primers with positive 585 bp amplicon. A total 26 Antibiotic disc used for antibiotic sensitivity test. Out of 26 Antibiotic disc, only two antibiotics (Imipenem and Cefoxitin) sensitive against isolated E. coli. Phenotypic characterization of ESBL by combine disk diffusion method. The difference between zone of inhibition is 20 mm around the combined disk containing clavulanic acid then the corresponding disk with Ceftazidime and cefotaxime is indicate positive for ESBL producing E. coli. Upon genotypic conformation of ESBL, the isolate was found positive for CTX-M1, CTX-M2, and TEM and negative for CTX-M9, SHV and OXA genes.
HIGHLIGHTS
Multi drug resistant ESBL producing E. coli was isolated from rabbit.
Harboring of CTXM-1, CTXM-2 and TEM genes and absence of CTX-M9, SHV and OXA genes were confirmed by PCR.
Escherichia coli, ESBL, Multidrug resistant, PCR, Rabbit
