1ICAR Research Complex for North Eastern Hill Region, Tripura Centre, Agartala, Lembucherra, West Tripura, India
2ICAR-Agricultural Technology Application Research Institute (ATARI), Indian Council of Agricultural Research, Bhumi Vihar Complex, Block-GB, Sector-III, Salt Lake, Kolkata, West Bengal, India
3Animal Biotechnology Centre, National Dairy Research Institute, Karnal, Haryana, India
4College of Fisheries, Central Agricultural University, Lembucherra, West Tripura, India
5Department of Veterinary Gynaecology and Obstetrics, College of Veterinary Sciences, GAD Veterinary and Animal Sciences University, Ludhiana, Punjab, India
*Corresponding author: A Haldar; E-mail: vetavijit@gmail.com
Online published on 27 May, 2021.
Anti-Mullerian hormone (AMH) is one of the important endocrine markers for the assessment of the age-related decline of fertility potential in animals. No baseline data is available on peripheral AMH concentrations at different ages in buffaloes (Bubalus bubalis). The present study thus aimed (i) to examine the responsiveness of the ovary to gonadotrophin releasing hormone (GnRH) challenge to release plasma AMH into circulation to address the question whether ovarian secretion of AMH would be affected by gonadotrophic status and (ii) to determine plasma AMH concentrations at different ages in buffaloes (n= 154) using an enzyme immune-assay. Data on hormonal concentrations in GnRH-treated buffaloes and in different ages of buffaloes were analyzed by non-parametric one-way repeated measure ANOVA and one-way ANOVA, respectively. No change (p>0.05) of plasma AMH concentrations after GnRH challenge in experimental buffaloes was recorded. Plasma AMH concentration was significantly lower (p<0.05) at 3 months of age (0.56 ± 0.29 ng/ml) and thereafter it started increasing and reached at a level of 3.07 ± 0.44 ng/ml at 3 years of age and then remained same (≥2 ng/ml) up to 11 years of age and again declined progressively and reached at the level of 0.52 ± 0.12 ng/ml at 15 years of age. A cubic model was the best fitted model to elucidate the change of plasma AMH level with age. Hence, the present study suggested that peripheral AMH concentration could be a candidate endocrine marker for the assessment of reproductive status in buffaloes.
HIGHLIGHTS
Age-specific peripheral anti-müllerian hormone concentration for the assessment of reproductive status in buffaloes.
Plasma AMH concentration is not influenced by hypothalamo-pituitary-gonadal axis or the gonadotropic status.
A cubic model fitted best the decline of plasma AMH concentration with age in buffaloes.
Anti-Mullerian hormone, GnRH challenge, Age-related change of AMH, Buffaloes
