Journal of Animal Research

  • Year: 2020
  • Volume: 10
  • Issue: 6

Polymorphism of the ovine calpastatin (CAST) gene and its association with productive traits in nellore sheep

  • Author:
  • B. Ramadevi1, B. Punya Kumari2*, K. Sudhakar3, G. Gangaraju4, U. Vinod2
  • Total Page Count: 7
  • Page Number: 881 to 887

1Animal Husbandry Polytechnic, Venkataramannagudem, Sri Venkateswara Veterinary University, Andhra Pradesh, India

2Department of Animal Genetics and Breeding, College of Veterinary Science, Sri Venkateswara Veterinary University, Tirupati-Andhra Pradesh, India

3Department of Animal Genetics and Breeding, College of Veterinary Science, Sri Venkateswara Veterinary University, Gannavaram-Andhra Pradesh, India

4Livestock Research Station, Palamaner, Chittoor, Andhra Pradesh, India

*Corresponding author: B PunyaKumari; E-mail: punya67@yahoo.co.in

Online published on 27 May, 2021.

Abstract

Calpastatin is a candidate marker for carcass and growth traits. The purpose of this work to study MspI polymorphism in the CAST gene in three strains of Nellore sheep breed viz., Jodipi, Brown and Palla and to find its relation with growth traits. The frequencies of M and N alleles in Nellore Jodipi, Nellore Brown and Nellore Palla were 0.83 and 0.17, 0.81 and 0.19, 0.76 and 0.24, respectively and genotype frequencies of MM, MN and NN genotypes were found to be 0.68, 0.31 and 0.01 in Nellore Jodipi; 0.64, 0.34 and 0.02 in Nellore Brown and 0.56, 0.40 and 0.04 in Nellore Palla, respectively. The PIC values for Nellore Jodipi, Nellore Brown and Nellore Palla are 0.24, 0.26 and 0.30 respectively, suggesting considerable amount of variation exist in these populations. Diversity estimates (FIS) were negative for the three populations of Nellore sheep i.e., Jodipi (−0.114), Brown (−0.105) and Palla (−0.097) indicating that there is no differentiation among the three populations. The χ2-test values revealed that all the three populations are in Hardy-Weinberg equilibrium. The association between genotypes and body weights revealed significant differences among three genotypes (MM, MN and NN) only for birth weight; while non-significant differences were observed among three genotypes for average daily gains.

HIGHLIGHTS

PCR RFLP technique was used to study the polymorphism of calpastatin gene in three strains of Nellore sheep.

Polymorphism of calpastatin gene significantly influenced the birth weight but not the average daily gains.

Keywords

Calpastatin, Hardy-Weinberg equilibrium, Msp1 Polymorphism and Nellore sheep