1Department of Veterinary Anatomy, College of Veterinary Science, SVVU, Proddatur, Andhra Pradesh, India
2Department of Veterinary Anatomy, College of Veterinary Science, SVVU, Tirupati, Andhra Pradesh, India
3NTR College of Veterinary Science, SVVU, Gannavaram, Andhra Pradesh, India
*Corresponding author: P Babu A; E-mail: prasanthankem@gmail.com
Online published on 27 May, 2021.
The present study was conducted in retina of 15 buffaloes and samples were categorized into group I (1–5 yrs), group II (6–10 yrs) and group III (10 yrs above). Immunopositive reaction for Pax6 in retinal pigment epithelium was strong in group I, mild to moderate reaction in group II buffaloes and no reaction in group III buffaloes. Strong immunopositive reaction against recover in was noticed in rods and cones, their nuclei of outer nuclear layer and binucleate cells of inner nuclear layer in group I and II buffaloes. Whereas moderate reaction was observed in the similar cells of retina in group III buffaloes. Strong Immunopositive reaction against calbindin was observed in the horizontal and amacrine cells of retina in group I and group II buffaloes and moderate reaction was observed in group III buffaloes.
HIGHLIGHTS
PAX6 reaction in retinal pigment epithelium was decreased with advancement of age.
Immunoreaction of recoverin in rod and cone cells was gradually decreased with advancement of age.
Calbindin activity in horizontall and amacrine cells of inner nuclear layer of retina was decreased in old animals.
PAX6, Recoverin, Calbindin, Immunoreactivity, Retina, Buffaloes
