1Department of Veterinary Parasitology, College of Veterinary Science & A.H., NDVSU, Jabalpur, Madhya Pradesh, India
2Department of Veterinary Pharmacology and Toxicology, College of Veterinary Science & A.H., NDVSU, Jabalpur, Madhya Pradesh, India
3Department of Veterinary Medicine, College of Veterinary Science & A.H., NDVSU, Jabalpur, Madhya Pradesh, India
*Corresponding author: G Das; E-mail: gdasvetpara@gmail.com
Online Published on 21 September, 2022.
The present investigation was carried out to evaluate the anthelmintic efficacy of crude aqueous and methanolic extract of Butea frondosa seeds extracts against benzimidazole resistant gastrointestinal nematodes of goats through in vitro and in vivo methods. In vitro investigation was carried out by egg hatch assay (EHA), larval paralysis test (LPT) and adult mortality test (AMT) against different stages of gastrointestinal nematodes whereas, in vivo by faecal egg count reduction (FECR) test in goats naturally infected with benzimidazole resistant GI nematodes. In in vitro trial, methanolic extract showed better ED50 in egg hatch assay and larval paralysis test as compared to aqueous extract. Moreover, in adult mortality tests, the methanolic extract gave better average corrected mortality as compared to aqueous extract. In vivo results revealed that the group treated with methanolic extracts showed the significant reduction (p<0.05) on 21st day whereas, the group treated with aqueous extract showed the highest and significant reduction (p<0.01) on 14th day. The results of both in vitro and in vivo trials suggest that B. frondosa possess anthelmintic activity and could be considered as one of the alternatives to the chemical anthelmintic.
• The methanolic and aqueous extracts of B. frondosa possess anthelmintic activity against the benzimidazole resistant GI nematodes of goats.
• Methanolic extract showed better efficacy as compare to aqueous extract.
Butea frondosa, Benzimidazole resistance, Anthelmintic activity, Gastrointestinal nematodes, Goat
