In Silico Characterization of ß-Defensin-1 Complementary Deoxyribonucleic Acid from Osmanabadi Goat

Beta-defensins are cationic antimicrobial peptides (AMPs) involved in resistance against microbial colonization. In this study total RNA was extracted from tongue of Osmanabadi goats and used to synthesize ß-Defensin-1 complementary deoxyribonucleic Acid (cDNA) using reverse transcriptase. In this study in silico characterization of Osmanabadi goat ß-Defensin-1 cDNA and its deduced peptide was carried out using PCR, Sequencing and DNAstar programme. CDS of Osmanabadi goat ß-Defensin-1 consists of 195 nucleotides encoding a protein of 64 amino acids. Multiple alignment at nucleotide level revealed that Osmanabadi goat ß-Defensin-1 had high homology with goat bD1-Y17679, Assam Hill goat Defensin, goat XM_018042143, and goat bD2-AJ009877 sequence. Similarly, multiple alignment at the amino acid level revealed that Osmanabadi goat ß-Defensin-1 had high identity with goat bD1-O97946, Assam Hill goat-AST23025, goat-XP_017897632 and goat bD2-097942 peptides. Phylogenetic tree analysis revealed that ß-Defensin-1 of Osmanabadi goat, both at nucleotide and amino acid level, is closely related to goat, sheep and Reindeer sequences. The Osmanabadi goat ß-Defensin-1 cDNA sequence is identical to goat ß-Defensin-1 reported from USA. No nucleotide substitution/ change was observed in Osmanabadi goat ß-Defensin-1 compared to ß-Defensin-1 of goat from USA. It also showed high percentage of identity with goat defensin-1 related molecules. Structure prediction using Phyre2 revealed goat ß-Defensin-1 had structural similarity with bovine neutrophil beta-defensin 12.

• Beta-defensins are cationic antimicrobial peptides (AMPs) involved in resistance against microbial colonization.

• In silico characterization of Osmanabadi goat ß-Defensin-1 cDNA