1Department of Veterinary Pathology, College of Veterinary Science, Assam Agricultural University, Khanapara, Guwahati, Assam, India
2Department of Animal Biotechnology, College of Veterinary Science, Assam Agricultural University, Khanapara, Guwahati, Assam, India
3Department of Veterinary Medicine, College of Veterinary Science, Assam Agricultural University, Khanapara, Guwahati, Assam, India
*Corresponding author: M. Buragohain; E-mail: mridusmritaburagohain@gmail.com
Online Published on 21 September, 2022.
In this study RT-PCR was used to detect CDV NP gene from the whole blood samples of dogs clinically suspected for CD followed by the study of pathomorphological alterations in different tissues. Clinical signs such as respiratory distress, purulent oculo-nasal discharge, biphasic fever, gastroenteritis, pustular dermatitis, prominent hyperkeratosis of the digital pads and nervous disorders were observed in the clinically suspected cases. The nervous disorder includes seizure, convulsion, paddling movement, tremor followed by posterior paralysis. On necropsy, bronchopneumonia, congestion and haemorrhages over the mucosal surface of the urinary bladder, stomach, intestine and brain were prominent lesions. Microscopically, both eosinophilic intranuclear and intracytoplasmic inclusion bodies were recorded in the neurons and glial cells, epithelial cells of the bronchioles, urinary bladder, kidney tubules and gastric glands. Congested vessels with haemorrhages were also found in the brain, lungs, stomach, intestine and lymphoid organs.
• Amplification of NP gene was found in 41.67 percent (n=25) cases out of total 60 sample tested by RT-PCR.
• Most prominent clinical manifestations were oculo-nasal discharge, gastroenteritis, biphasic fever, hyperkeratosis, pustular dermatitis and nervous disorders.
• In histopathological studies inclusion bodies were recorded in the lungs, brain, kidneys, stomach and urinary bladder.
Canine distemper virus, Dog, Histopathology, RT-PCR
