Journal of Animal Research
  • Year: 2021
  • Volume: 11
  • Issue: 3

Detection of Antimicrobial Resistance in Escherichia coli and Salmonella Isolated from Flies Trapped at Animal and Poultry Farm Premises

  • Author:
  • Bhavna Wadaskar1, Rahul Kolhe1,*, Vikas Waskar1, Mrunalini Budhe2, Krishnendu Kundu3, Sandeep Chaudhari4
  • Total Page Count: 10
  • Published Online: Sep 22, 2022
  • Page Number: 341 to 350

1Department of Veterinary Public Health, KNP College of Veterinary Science, Shirwal, District Satara, Maharashtra Animal & Fishery Sciences University, Nagpur, India

2Department of Veterinary Microbiology, KNP College of Veterinary Science, Shirwal, District Satara, Maharashtra Animal & Fishery Sciences University, Nagpur, India

3Department of Veterinary Parasitology, KNP College of Veterinary Science, Shirwal, District Satara, Maharashtra Animal & Fishery Sciences University, Nagpur, India

4Department of Veterinary Public Health, Nagpur Veterinary College, Seminary Hills Nagpur, Maharashtra Animal & Fishery Sciences University, Nagpur, India

*Corresponding author: RP Kolhe; E-mail: dr_kolherahul@rediffmail.com

Online Published on 22 September, 2022.

Abstract

The aim of this study was to explore antimicrobial resistance in Escherichia coli and Salmonella species from flies trapped at livestock and poultry farm premises. A total of 36 pools of flies and 72 rectal/cloacae swabs were collected. All the flies were Musca domestica except one fly was Calliphora erythrocephala. E. coli were recovered from all the flies (100%) and fecal (100%) samples. Whereas, Salmonellae were obtained from 21 (58.33%) flies and 15 (20.83%) fecal samples. E. coli and Salmonella isolates were multi-drug resistant strains. E. coli exhibited resistance to amoxicillin-clavulanic acid (100%), cefotaxime (93.57%), aztreonam (59.63%), cefpodoxime (58.71%) and imipenem (48.62%). Salmonellae were also 100% resistant to ampicillin-clavulanic acid followed by cefotaxime (91.66%), cefpodoxime (94.44%) and imipenem (91.66%). Colistin resistance was recorded more in Salmonella (61.11%) than E. coli (12.84%) by phenotypic assays, however, mcr1 to mcr5 genes could not be detected in any of the E. coli and Salmonella isolates. Bacteria studied were ESBL (21.10%) and MBL positive. Present study is suggestive of the fact that flies harbor multidrug resistant, ESBL, MBL and colistin resistant E. coli and Salmonella strains. Extensive monitoring of indicators organisms of AMR in unconventional reservoirs like flies is needed.

• Study focused on the detection of AMR in E. coli and Salmonella present in the flies.

• Musca domestica is highly prevalent at livestock and poultry farm premises.

• Flies harbor multidrug resistant, ESBL, MBL and colistin resistant E. coli and Salmonella strains in their gut.

Keywords

E. coli, Salmonella, AMR, Flies, Food animals, Poultry