1Animal Physiology and Reproduction Division, ICAR-Central Institute for Research on Buffaloes, Hissar, Haryana, India
2Department of Veterinary Gynaecology and Obstetrics, Rajasthan University of Veterinary and Animal Sciences, Bikaner, Rajasthan, India
3Department of Animal Breeding and Genetics, Rajasthan University of Veterinary and Animal Sciences, Bikaner, Rajasthan, India
*Corresponding author: Satish; E-mail: satishnain26@gmail.com
Online Published on 13 July, 2023.
With an objective to evaluate the value of different extenders semen was collected from Murrah buffalo bulls (n=6) by artificial vagina and subjected to dilution using different extenders viz Tris egg yolk citrate (TEYC), LDL, tocopherol (TEYC with 1 mg/mL tocopherol) and sericin (TEYC with 0.25% sericin) based extenders. Semen was cryopreserved and sperm with intact acrosome were evaluated by flow cytometry immediately after dilution, at equilibration stage and after cryopreservation. There were non-significant differences in the proportion of live sperm with intact acrosome (frozen-thawed semen) for all the diluters studied except for buffalo bull number 5333 which evidenced significantly lower (P>0.05) proportion of live sperm with intact sperm in the tocopherol based extender. The overall percent live sperm with intact sperm was significantly higher (P<0.001) for LDL based extender as compared to TEYC, tocopherol, and sericin extenders at the equilibration stage and in frozen-thawed semen. It was concluded that the LDL based extender is best for cryopreservation of buffalo semen.
• Sperms with intact acrosomes are not significantly different at three stages of cryopreservation.
• Tris supplemented with LDL yields the highest percent live sperms with intact acrosome.
Acrosome, Buffalo, LDL, Flow cytometry, Sericin, Vitamin E
