College of Veterinary Science & Animal Husbandry, Anjora, Dau Shri Vasudev Chandrakar Kamdhenu Vishwavidyalaya, Durg, Chhattisgarh, India
*Corresponding author: C Sannat; E-mail: drsannat@gmail.com
Online Published on 27 December, 2023.
The present study was conducted to characterize Staphylococcus aureus and methicillin-resistant Staphylococcus aureus (MRSA) in mastitic milk of cow using rapid and cost effective genotypic as well as phenotypic method. Different phenotypic and genotypic methods were compared in detection of S. aureus and MRSA. Present study also optimized and standardized the boiling and snap chilling method of genomic DNA extraction for rapid screening of S. aureus and MRSA in pure culture. Detection of species specific thermo nuclease (nuc) gene was found more sensitive than phenotypic test in identification of S. aureus whereas both rapid cultural methods such as cefoxitin disc diffusion test and use of chromogenic agar was found comparable to mecA gene based PCR in characterization of MRSA. Furthermore, despite less purity and minute concentration of DNA obtained from boiling and snap chilling method, there was no non-specific and cross reaction in PCR assay. Therefore the boiling and snap chilling method could be recommended as an alternative and economical method of genomic DNA extraction for rapid screening of S. aureus and MRSA particularly in developing countries like India.
• Boiling and snap chilling was found economic method of genomic DNA extraction for screening of Staphylococcus aureus and MRSA associated genes.
• Cefoxitin disc diffusion test and chromogenic MeReSa agar were observed as good as mecA gene based PCR for screening of MRSA.
Staphylococcus Aureus, Nuc gene, MRSA, MecA gene, Boiling and Snap Chilling Method
