Department of Veterinary Microbiology, College of Veterinary and Animal Science, Navania, Vallabhnagar, Udaipur, Rajasthan University of Veterinary and Animal Sciences Bikaner, Rajasthan, India
*Corresponding author: DK Sharma; E-mail: ds132207@gmail.com
Online Published on 30 December, 2022.
Chicken parvovirus (ChPV) is of many viruses related to enteric diseases, which usually associated with enteritis and Runting and stunting syndrome (RSS). Aim of the present study was to find out the occurrence of ChPV associated with poultry enteritis in four districts of southern Rajasthan, and their phylogenetic analysis. Total 604 dead poultry birds affected with enteritis, from commercial poultry farms were examined for presence of ChPV. Intestinal samples of four birds were pooled to make one biological sample. ChPV were detected by PCR using non-structural protein (NSP) gene specific primers. Phylogenetic analysis was carried out by partial gene sequencing. ChPV was detected in 18.54% (28/ 151). Mixed infection of FAdV+ChPV were detected in 15.23% (23/151) pooled samples. The partial molecular characterization of the ChPV partial molecular characterization revelled high level of nucleotide 98.95-100% and amino acid similarity 98.95-99.98% with other ChPV sequences from Brazil and other parts of the world.
• ChPV was detected in 33.77% (51/ 151) in pooled samples from four districts of Southern Rajasthan.
• Hhigh level similarity was found with other ChPV sequences from Brazil and other parts of the world.
Chicken Parvovirus, PCR, Poultry Enteritis, Phylogenetic Analysis
