Management of subclinical endometritis by immunomodulators and proteolytic enzymes

Five hundred repeat breeder cows (2-4 parity) were randomly selected from livestock farm, Kuthuliya and villages of Rewa (M.P.). On the basis of endometrial cytology by cytobrush technique 140 repeat breeder cows (28%) were diagnosed to be suffering from subclinical endometritis. Out of the animals found positive 30 cows were selected and were divided into 5 groups (n=06 per group). The group wise treatment allotted were Group I-Levamisole, 2.5 mg/kg body weight, once for three weeks, S/c; Group II-100 µgm E. coli LPS in 30 ml PBS, single I/U infusion; Group III-500 mg Benzathine cephapirin, single I/U infusion; Group IV-Proteolytic enzymes in 10 ml distilled water, single I/U infusion and Group V (Control)-no treatment. All the animals were subjected to endometrial cytology at 12 hrs., 24 hrs. and 48 hrs. post-treatment. The animals were bred at next successive oestrus and pregnancy was confirmed 60 days post insemination. Endometrial cytology in treatment group I revealed that the PMN per cent increased significantly (p<0.05) from 0 hrs. to 24 hrs. In groups II, III and IV, the increase in PMN per cent was significant (p<0.05) from 0 hrs. to 48 hrs. The overall pregnancy rate was higher (83.33%) in treatment groups II and IV as compared to treatment group III (50.00%), group I (33.33%) and group V (0.00%), respectively. It was concluded that immunomodulators like E. coli lipopolysaccharide and proteolytic enzymes proved to be better for enhancing uterine immunity and pregnancy rates in subclinical endometritic repeat breeder cows.