Hematological and serum biochemical evaluation of doxorubicin induced toxicity in wistar male rats and its amelioration with hesperidin

Doxorubicin (DOX), an anthracycline antibiotic is used successfully to treat a variety of cancers. The present study was designed to evaluate protective role of hesperidin, a flavanone glycoside, against doxorubicin induced toxicity. For this purpose, 30 adult male Wister rats were divided into five different groups consisting six in each group. Normal saline was given to the group I as sham. Dose of 200 mg/kg of HES to the group III was given orally for 2 weeks. Group II was kept as DOX control (2.5 mg/kg body weight) four times in a week, intraperitoneally for 2 weeks. Group IV and V, were administered hesperidin low dose (100 mg/kg body weight) and high dose (200 mg/kg body weight), respectively, orally with DOX four times in a week over a period of two weeks. At the end of the experiment, hematological and biochemical parameters were performed in blood of rats. Results showed that DOX caused a marked rise in biochemical parameters such as serum creatinine kinase-mycocardial band, serum lactate dehydrogenase, alkaline phosphatase , troponins, serum glutamic oxaloacetic transaminase and serum glutamic pyruvic transaminase activities alongside an increase in serum total cholesterol and triglycerides level, and decrease in the values of hematological parameters such as total erythrocytes count, total leukocytes count, hemoglobin and pack cell volume. However, hesperidin low and high dose treated group IV and V, respectively exhibited significant (<0.05) improvement in all the above parameters as compared group II indicating the protective role of hesperidin.