Isolation of lumpy skin disease virus from Andhra Pradesh

A total of 31 blood samples and 31 skin scabs were collected from both adult and young calves of suspected cases of Lumpy skin disease showing clinical signs. All the collected samples (31 blood samples and 31 skin scabs) were processed and subjected for virus isolation in embryonated chicken eggs and primary testicular cell cultures. The skin scabs (7 out of 14) positive at genus and (amplifying P32 gene) species level amplifying (GPCR, RPO30 and Ankyrin repeat) genes by PCR were attempted for virus isolation on embryonated chicken eggs of 10–11 days old. All the seven virus isolates (TPT-1, TPT-2, K-1, K-2, G-1, CH-1, CH-2) showed hemorrhages on chorio-allantoic membrane at second passage level on 5^th day of post inoculation whereas only three isolates (TPT-2, K-1, CH-1) showed characteristic pock lesions on 5^th day of post inoculation at 5^th passage level. These were further confirmed by PCR amplifying P32 gene. Out of these three isolates (TPT-2, K-1, CH-1) only two isolates (TPT-2, K-1) were subjected for adaptation in primary lamb testicular cell cultures and CPE was observed with shrinkage, cell rounding and cell aggregation between 48–72hrs post infection at first blind passage level.

⓿ Isolation of Lumpy skin disease virus from Blood and Skin scabs.

⓿ Isolation of Lumpy skin disease virus in Embryonated chicken eggs and primary testicular cell cultures.