Journal of Animal Research
  • Year: 2012
  • Volume: 2
  • Issue: 2

Detection of P1 type of bovine rotavirus using nested multiplex PCR in Mathura, India

  • Author:
  • Anuj Tewari1,2,, Sandeep Kumar Dash2, Beenu Jain2, Ashok Kumar Bhatia2
  • Total Page Count: 7
  • Page Number: 179 to 185

Department of Veterinary Microbiology & Immunology College of Veterinary Sciences and Animal Husbandry, DUVASU, Mathura, India.

Present address: 1Anuj Tewari, Mathura Niwas, 11 Awadhpuri extension, Sarvodaya nagar, Lucknow, (UP), India.

2Department of Veterinary Microbiology & Immunology

*Corresponding Author: anuj474@gmail.com

Online published on 31 August, 2012.

Abstract

One hundred twenty seven (127) diarrheic fecal samples collected from dairy farms of Mathura, India were screened for rotaviruses by RNA-Polyacrylamide gel electrophoresis (RNA PAGE). These were further characterized on the basis of P types (VP4 gene) by a nested multiplex Reverse transcriptase Polymerase chain reaction (RT-PCR) assay. P typing revealed the presence of the P1 type in two samples yielding a product size of 463 bp an indicator of P1 type. This paper also describes the attempt to isolate the rotavirus in cell culture. The present study reveals the presence of P1 type of bovine rotavirus in Mathura, India.

Keywords

Bovine rotavirus, Nested multiplex Reverse transcriptase Polymerase chain reaction (RT-PCR), P1 type