1Department of Veterinary Physiology, College of Veterinary Science, Assam Agricultural University, Guwahati, Assam, India
2Division of Veterinary Physiology and Biochemistry, Faculty of Veterinary Sciences and Animal Husbandry, Sher-e-Kashmir University of Agricultural Sciences and Technology-Jammu, R.S. Pura, Jammu & Kashmir, India
3Division of Veterinary Pathology, Faculty of Veterinary Sciences and Animal Husbandry, Sher-e-Kashmir University of Agricultural Sciences and Technology-Jammu, R.S. Pura, Jammu & Kashmir, India
*Corresponding author: jafrinahmed@rediffmail.com
Online published on 19 November, 2015.
The present study was undertaken to evaluate the aspiration method for recovery of cumulus oocyte complexes (COCs) from bovine ovaries at slaughter. A total of 829 cattle ovaries were collected from slaughter house, out of which 2,935 COCs were aspirated out. The mean number of COCs recovered was 3.54 per ovary. The COCs recovered were graded variously as A, B, C and D grades. Grade ‘A’ and ‘B’ COC's were 38.77% and 27.02% respectively, and the average culture grade (Grade A and B COC's together) recovery was 65.79%, representing 2.33 COCs per ovary. The number and percentage recovery of A and B grade COCs were higher than that of C (18.13%; 0.64/ovary) and D (16.08%; 0.57/ovary) grades. The aspiration method adopted in the present study was found to be efficient for recovery of ample amount of COCs and yield good quality culturable oocytes for subsequent in vitro studies. The aspiration method also allows greater visual assessment of follicles, their selection and assortment for quality COC yield.
Aspiration, bovine, cumulus oocyte complexes, ovaries
