Evaluation of PPD based ELISA in the Diagnosis of Bovine Tuberculosis

541 animals from three dairy farms () were firstly screened for bovine tuberculosis by tuberculin skin testing, out of which 71 (13.12%) animals were found tuberculin reactors. The serum samples of 71 tuberculin positive-104 tuberculin negative and 363 non tuberculin tested animals were then evaluated by Purified Protein Derivative (PPD)-ELISA. PPD-ELISA yielded 57.74%, 8.65% and 24.24% seropositivity in tuberculin positive, tuberculin negative and non-tuberculin tested animals with an overall seroprevalence of 25.65% among tested sera. The relative sensitivity and specificity of ELISA with tubercul in test was 57.74% and 91.34%, respectively. The use of PPD based ELISA may be suggested in conjugation with tuberculin test for whole herd screening and culling programme especially in anergic state or advanced stages of infection.