Single Nucleotide Polymorphism in the 5′-Upstream Region of Activin A Receptor, Type IIB in Aseel Chicken

Activin A receptor, type IIB (ACVR2B) is a protein that is encoded by the ACVR2B gene, which plays a crucial role during muscle development in chicken. The present investigation was conducted to ascertain the nucleotide variability in the 5′-upstream regulatory region of ACVR2B and its association with body weight at different ages in Aseel chicken. Polymerase chain reaction single-strand conformation polymorphism (PCR-SSCP) analysis of the regulatory region revealed two novel mutations (T68A and C172T) in the 5′-untranslated region of ACVR2B. However, both the mutations observed were common to all the birds involved in the current study, indicating that 5′-upstream regulatory region of ACVR2B gene was monomorphic in Aseel. Being monomorphic, no relationship was found with growth traits in Aseel. Absence of polymorphism in the regulatory region suggests that the regulation pattern of ACVR2B gene might be consistent with all birds in this region.