Efficacy of Indirect Enzyme Immunosorbent Assay and Passive Haemagglutination Test for the Diagnosis of Bovine Herpes Virus-1 (BHV-1) Infection

A total of 255 sera were collected from unvaccinated crossbred (174) and indigenous (81) cattle and buffalo having history of respiratory and reproductive disorders. All the sera sample were subjected to indirect ELISA and passive haemagglutination (PHA) test for the detection of BHV-1 antibodies. Indirect ELISA and PHA test detected significantly (P<0.01) varied prevalence of BHV-1 antibody i.e. 60.39% and 35.69%, respectively. The sensitivity and specificity of PHA test in comparison with indirect ELISA was 55.19% and 94.06%, respectively. Moderate agreement (kappa = 0.442) was noticed between ELISA and PHA for the diagnosis of BHV-1 antibodies. Significantly (p< 0.01) higher prevalence of BHV-1 antibodies was observed in crossbred (70.11%) as compared to indigenous cattle and buffalo (46.91%). Hence, it is be concluded from present investigation that the indirect ELISA could be reliable and sensitive test than PHA test to screen BHV-1 antibody.