Kinematic Response of Barbari and Sirohi Buck Spermatozoa to High Egg Yolk Concentration in Semen Extender

The experiment was designed to evaluate kinematic characteristic exhibited by spermatozoa in diluted Barbari and Sirohi buck semen using computer assisted semen analysis. Four normal healthy adult bucks (2 Barbari and 2 Sirohi) reared at the experimental goat sheds of department were selected for semen collection. Semen was collected twice a week using artificial vagina. A total of 8ejaculates (sixteen ejaculates from each breed) were collected from each buck. After initial semen evaluation, ejaculates from similar breed were pooled. The pooled semen sample of two breeds were dilutedwith TRIS based extender containing 20 per cent egg yolkto final concentration of 100×10⁶ spermatozoa per ml. Motility patterns and path velocities of spermatozoa were evaluated using computer assisted semen analyser (CASA). It was observed that proportion of spermatozoa exhibiting rapid progression were highest in both the breeds, following by slow progression and non-progression. A significantly (p<0.01) higher values of non-progressive spermatozoa were observed in Barbari buck semen. Different path velocities viz. VCL (μm/sec), VAP (μm/sec), VSL (μm/sec), Lin (%), Str(%), WOB(%), BCF(hz), ALH(μm) and DNC(μm²/sec) exhibited by spermatozoa were recorded during the experiment. The VCL (μm/sec), ALH(μm) and DNC(μm²/sec) were significantly(p<0.01, p<0.05) in Barbari buck semen while a significantly(p<0.01, p<0.05) higher values of VSL, Lin (%), Str (%), WOB(%)and BCF (hz) were observed for Sirohibuck. The result indicated that Barbari buck spermatozoa covered more distance per unit time in the vertical direction (Y-axis) and short X axial movements (horizontal movement), while the spermatozoa of Sirohi Buck moved at slower velocity with more distance covered in horizontal direction (X axis) and lesser vertical displacementresulting in lower values of VCL and higher values of VAP and VSL as compared to Barbari buck. So, it can be concluded that a variable response is exhibited by Barbari and Sirohi buck spermatozoato egg yolk concentration, indicating a breed specific variation in motion characteristic and path velocities of spermatozoa.