1Department of Epidemiology and Preventive Medicine, College of Veterinary and Animal Science, G.B. Pant University of Agriculture and Technology, Pantnagar, Udham Singh Nagar, Uttarakhand, India
3Virology Laboratory, Centre for Animal Disease Research and Diagnosis (CADRAD), Indian Veterinary Research Institute (IVRI), Izatnagar, Bareilly, Uttar Pradesh, India
*Corresponding author: V Thakur; Email: vipulthakur29153@gmail.com
Online published on 4 November, 2016.
The present study was carried out to make a comparative evaluation of a commercial competitive ELISA kit with respect to gold standard micro-serum neutralization test (mSNT) for detection of antibodies to Bovine herpes virus-1. The relative sensitivity and specificity of cELISA were found to be 96.67% and 95.29%, respectively. The positive and negative predictive values were observed to be 78.38 and 95.29%, respectively. The exhibited kappa value was 0.95. From this study, it is evident that cELISA is very simple, rapid with high specificity and sensitivity in detecting BHV-1 antibodies and can prove to be a quick and effective tool in diagnosis of the disease in field conditions where mSNT cannot be carried out due to its inherent requirement of highly specialized laboratories with cell culture facilities and expert personnel.
cELISA, mSNT, antibodies, BHV-1
