1All India Institute of Hygiene and Public Health, Kolkata, India
2Temperate Animal Husbandry, Indian Veterinary Research Institute, Mukteshwar, India
3Sher-e-Kashmir University of Agricultural Sciences and Technology of Jammu, India
4Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, India
*Corresponding author: R Singh; Email: drraghubir166@gmail.com
Online published on 7 February, 2017.
The present study dealt with cloning and molecular characterization of MI CIO gene ofT. gondii Kolk ata isolate TgM CIO is a small microneme protein that lacks a putative transmembrane domain and appears not to remain associated with the parasite membrane after micronemal discharge. MI C10 gene ol gondii "Kolk ata isolate was PCR amplified and cloned into a TA cloning vector (Promega) to facilitate sequencing and subsequent characterization. The product revealed 597bp in size and has 99.2% homology with the published sequence. The difference was observed at 22, 210, 235, 378 and 513 positions. When the gene was deduced for amino acids, the homology was 98% with published sequence. The total amino acids number was 198 with predicted mass of 23 kDa molecular weight. The changes were observed at 8, 115 and 171th amino acid positions.
Toxoplasma gondii, MI C10, TA cloning vector
