Department of Veterinary Gynaecology & Obstetrics, College of Veterinary Science and Animal Husbandry, U.P. Pandit Deen Dayal Upadhyaya Pashu Chikitsa Vigyan Vishvavidyalya Evam Go Anusandhan Sansthan, Mathura, India
*Corresponding author: A Patel; Email: dr.akhil.patel87@gmail.com
Online published on 7 February, 2017.
The present study was aimed to see the effect of butylated hydroxytoluene (BHT) as semen additives during cryopreservation of Hariana Bull semen. Twenty four ejaculates from two Hariana bulls extended with glycerolated egg yolk tris (GEYT). Extended samples were split into three aliquots. One aliquot was kept as control while other two aliquots were supplemented with 0.5 mM (Tl) and 1.0 mM (T2) of BHT. All semen samples were equilibrated and cryopreserved in liquid nitrogen vapours and subsequently plunged in liquid nitrogen for preservation. The effect of BHT was determined by assessment of sperm viability and acrosomal integrity at three stages i.e. after dilution, pre-freeze and post-thaw. Our study revealed that addition of 1.0 mM of BHT results in a significant (p<0.05) improvement in sperm viability and acrosomal integrity during cryopreservation.
acrosomal integrity, butylated hydroxyltoluene, Hariana bull, sperm and viability
