Journal of Animal Research
  • Year: 2017
  • Volume: 7
  • Issue: 2

Molecular Prevalence of Hepatozoon canis Infection in Dog Tick, Rhipicephalus sanguineus, from Punjab, India

  • Author:
  • Kaushlendra Singh1, Harkirat Singh1,, N.K. Singh1, N. Kashyap2, S.S. Rath1
  • Total Page Count: 4
  • Page Number: 401 to 404

1Department of Veterinary Parasitology, College of Veterinary Science, Guru Angad Dev Veterinary and Animal Sciences University (GADVASU), Ludhiana, Punjab, India

2Department of Animal Genetics & Breeding, College of Veterinary Science, Guru Angad Dev Veterinary and Animal Sciences University (GADVASU), Ludhiana, Punjab, India

*Corresponding author: H Singh; Email: drharkiratsingh@hotmail.com

Online published on 15 June, 2017.

Abstract

Canine hepatozoonosis, caused by Hepatozoon canis, is transmitted by the ingestion of brown dog tick, Rhipicephalus sanguineus. In the present study R. sanguineus ticks (n=60) collected from dogs presented at Small Animal Clinics, Teaching Veterinary Clinical Complex, GADVASU, Ludhiana, Punjab, India were screened by PCR based assay for the detection of H. canis specific 18S ribosomal RNA gene fragment. Results of the PCR assay revealed an overall prevalence of 8.33% H. canis infection in the tested ticks. Furthermore, the male ticks showed higher prevalence (11.53%) than the female counterparts (5.88%) but the data was statistically non-significant (P=0.6439).

Keywords

Dog tick, Hepatozoon canis, PCR, Punjab, Rhipicephalus sanguineus