1Department of Veterinary Biochemistry, College of Veterinary Science and Animal Husbandry, DUVASU, Mathura, India
2Department of Animal Genetics and Breeding, College of Veterinary Science and Animal Husbandry, DUVASU, Mathura, India
*Corresponding author: V Pandey; Email: drvijaypandey@gmail.com
Online published on 19 July, 2017.
Leptin gene is considered as marker of production and reproduction traits in dairy or beef cattle. The aim of this study was to investigate the genetic polymorphism in LEP gene in Hariana cows and their associations with reproduction traits. The blood samples were collected from 62 Hariana cows and analyzed in order to identify LEP/BsaAI (BTA 4, intron 2 and exon 3) genotypes using PCR-RFLP method. The allele frequencies observed were 0.37 and 0.63 for A and B LEP variants and genotypic frequencies were 9.67, 54.8 and 35.5 for AA, AB and BB genotype variants, respectively. Statistical analysis showed that SNP LEP/BsaAI significantly affected gestation period and dry period in analyzed population of cows. Observations of this investigation advocated that leptin is a candidate gene, which affects reproduction traits and might be implemented in breeding strategies to improve the reproductive performance of Hariana cattle breed.
Hariana cows, leptin gene, reproduction, polymorphism
