Sensitivity of Multiplex PCR Assay for Selected Animal Species

Multiplex PCR assay was quick, simple and sensitive method for identification multiple meat species in single PCR reaction. Mitochondrial cytochrome b gene was used as an indicator gene for identification of selected animal species (Buffalo, Sheep, Goat and Chicken) by using multiplex PCR. Sensitivity of the multiplex PCR assay was determined by using 100 ng/μl DNA of meat of Buffalo, Sheep, Goat and Chicken. For the estimation of sensitivity of multiplex PCR assay (34 cycles) made subsequently 10 fold dilution of 100 ng/μl DNA i.e. 100, 10, 1, 0.1, 0.01, 0.001 ng/μl and used in multiplex PCR reaction. In present study the lowest sensitivity of the multiplex PCR assay for all the four selected animal species DNA concentration was fined up to 0.01 ng/μl.