1Department of Animal Genetics and Breeding, College of Veterinary Science and A.H, Mhow, Indore, Madhya Pradesh, India
2Department of Veterinary Biochemistry, College of Veterinary Science and A.H, Mhow, Indore, Madhya Pradesh, India
*Corresponding author: S Joshi; Email: joshi.sk.vet@gmail.com
Online published on 19 July, 2017.
Fifty Holstein Friesian (H.F) cattle were screened for Bovine Citrullinaemia (BC) using PCR-RFLP. Blood samples were collected from jugular vein in 2 ml capacity vaccutainers (K2 EDTA) and the DNA was isolated by using whole blood extraction kit. The PCR was performed for amplification of polymorphic region of ASS gene (185 bp) on bovine chromosome 11. The PCR products were digested with Ava II endonuclease enzyme. The normal allele in unaffected cattle produced two fragments of 103 bp and 82 bp. No animal was found carrier for ASS gene. The genotype frequency of normal individuals and the gene frequency of normal allele were found to be one.
Bovine Citrullinaemia (BC), holstein friesian cattle, ASS gene, PCR-RFLP
