Efficacy of Microtiter Method of Direct Antiglobulin Test in diagnosis of Immune Mediated Haemolytic Anaemia in Dogs

The present study was carried out to assess the efficacy of microtiter method of direct antiglobulin test in diagnosis of Immune Mediated Haemolytic Anaemia in Dogs. Two hundred and fifty eight anaemic dogs with pale/icteric mucous membrane were used for the present study. Initial screening of these dogs was done with saline agglutination test. Forty seven dogs were found positive for saline agglutination test. Whole blood samples of these dogs were used for direct antiglobulin test (DAT). The test was performed using polyvalent antiserum in a 96-well microtiter plate allowing multiple dilutions of antisera. Twenty five dogs were found to be positive for DAT at various titers. The use of microtiter method helped in detecting 8 additional Coombs’ positive dogs which would be negative in standard tube method dilution. The use of this method increases the sensitivity of DAT by overcoming prozone effect.