Journal of Animal Research
  • Year: 2017
  • Volume: 7
  • Issue: 4

Absence of NsiI Polymorphism in Growth Hormone Receptor (GHR) Gene in Indian Cattle Breeds

  • Author:
  • Gaurav Parihar1, Deepak Sharma1, Satyendra Pal Singh1,, Madhu Tiwari1, Rakesh Goel1, Sanjeev Kumar Singh2, Vijay Pandey3
  • Total Page Count: 3
  • Page Number: 661 to 663

1Department of Animal Genetics and Breeding, C.o. V. Sc. & A.H., DUVASU, Mathura, India

2Department of Veterinary Extension, Co. V. Sc. & A.H., DUVASU, Mathura, India

3Department of Veterinary Biochemistry, C.o. VSc. & A.H., DUVASU, Mathura, India

*Corresponding author: SP Singh; Email: satsinpal21@gmail.com

Online published on 9 October, 2017.

Abstract

The objective of the present study was to investigate the polymorphism in 5’ non-coding region of growth hormone receptor (GHR) gene in Sahiwal (n = 53) and Hariana (n = 50) cattle using Nsi I/PCR-RFLP assay. Amplifcation of DNA sample revealed 302bp product using specifc primer pairs and digested by using NsiI restriction enzyme. All the screened animals were found monomorphic in nature for GHR/NsiI polymorphism. It revealed that only one type of uncut banding pattern (AA genotype); which was of 302bp. We could not identify any animal with GG and AG genotypes. Consequently, we could not perform association studies with milk production traits.

Keywords

Sahiwal, Hariana, PCR-RFLP, GHR, NsiI Polymorphism, Milk production traits