Method Validation for Analysis of 24 Pesticides in Catla catla fish through Gas Chromatography Triple Quadrupole Mass Spectrometer (GC-MS/MS)

A multiresidue method was developed and validated for 24 pesticide (Organo chlorine, Organophophorus and Synthetic pyrethroids) residue in Catla fish sample using Gas Chromatography Triple Quadrupole Mass Spectrometer (GC-MS/MS). The method was based on QuEchERs method (quick, easy, cheap, efficient, rugged and safe) extraction with acetonitrile and dispersive solid phase extraction (d-SPE) cleanup with PSA and MgSO₄. The recovery studies were conducted at three spiking levels i.e. 0.01, 0.05 and 0.1 μg mL⁻¹ and percent recovery found to be within acceptable limit except synthetic pyrethrnoids at spiking level 0.01 μg mL⁻¹. All RSD values were below 20%. The estimated LOD was in the range from 0.000–0.017 μg mL⁻¹ and LOQ was in the range from 0.001–0.057 μg mL⁻¹ for all pesticides. The coefficient of determination (R²) for all pesticide ≥ 0.992. The devlop method was found to be fast, simple and reliable for analysis of pesticide residue in fish samples.