1Department of Veterinary Public Health, DUVASU, Mathura, Uttar Pradesh, India
2Department of Veterinary Epidemiology and Preventive Medicine, DUVASU, Mathura, Uttar Pradesh, India
3Department of Livestock Products Technology; (DUVASU), Mathura, Uttar Pradesh, India
4Department of Pharmacology and Toxicology; (DUVASU), Mathura, Uttar Pradesh, India
*Corresponding author: RP Mishra; Email: rmishra523@rediffmail.com
Online published on 7 December, 2018.
The study was undertaken for detection of virulence gene using polymerase chain reaction (PCR) and investigate their antibiotic sensitivity pattern in fecal sample of buffalo. In present study, a total of 180 samples were processed for isolation of verocytotoxic E. coli (VTEC). Out of 180 samples, 19 samples were found positive for VTEC. All VTEC strains were subjected to antibiotic sensitivity test using 16 antibiotic discs. Of these, ciprofoxacin and imipenem were found highly sensitive (100%) against VTEC strains followed by ceftriaxone (89.47%), gentamicin (84.21%), amikacin (84.21%) and chloramphenicol (78.94%). Least sensitivity was recorded for enrofloxacin (78.94%), followed by cefixime (63.15%), erythromycin (57.84%) and tetracycline (52.63%).
Antibiotic sensitivity test, Vercytotoxic E. coli. faecal sample, PCR
