1Department of Veterinary Medicine, Rajiv Gandhi Institute of Veterinary Education and Research, Pondicherry, India
2Department of Biochemistry, Rajiv Gandhi Institute of Veterinary Education and Research, Pondicherry, India
*Corresponding author: K Rajkumar; Email: rajvet10@gmail.com
Online published on 7 December, 2018.
A work was conducted during the months of January-August 2014 in Pondicherry in order to find the species of Theileria prevalent in the cross bred and Non-descript cattle. The selection of cases was based on the clinical signs and confirmative diagnosis by Polymerase Chain Reaction (PCR) for genus and species specific. The PCR analysis of the bovine blood revealed that 100 out of 110 animals were infected with Theileria, while seventy eight of those 100 samples were positive for T. orientalis. The primers used for detection of Theileria specific was Small Sub-unit r-RNA (SSrRNA) primer of 1098 bp and for T. orientalis- Major Piroplasm Surface Protein (MPSP) primer of 849 bp.
PCR, T. orientalis, cattle, Pondicherry
