Effect of Dietary Cholesterol and Niacin Supplementation on Serum Enzymatic Alterations in Experimentally Induced Renal Dysfunction in Wistar Rats

Study was carried out to evaluate the effect of dietary cholesterol and niacin supplementation and renal dysfunction in wistar rats. Eighty-four adult healthy wistar male rats were divided into twelve equal groups. Experiment was carried out as per 2 × 3 × 2 factorial design with two clinical conditions (Kidney-Normal and Kidney-Compromised); three levels of cholesterol supplementation (0%, 0.5% and 1.0%) and two niacin levels (unsupplemented and supplemented @ 100 mg/kg body weight), respectively. At the start of the experiment, renal dysfunction was induced in respective rats by daily injection of gentamicin for 8 days. Blood samples were collected from experimental animals on zero, 20^th, 40^th and 60^th days of trial to study liver specific serum enzyme profile viz aspartate amino transaminase (AST), alanine amino transaminase (ALT), alkaline phosphatase (ALP) and acid phosphatase (ACP).Gentamicin injection resulted in significantly increase in levels of enzymes. Also cholesterol supplementation @ 0.5% and 1% resulted in significantly higher levels of enzymes studied. However, treatment with niacin @ 100 mg/kg body weight resulted in marked improvement in level of enzymes studied.