Effect of Dilution and Sperm Concentration on Post Thaw Semen Quality in Barbari Buck

Deleterious interaction between seminal plasma proteins and egg yolk has been well documented in buck semen. Reduced lethal contact between seminal proteins and egg yolk can be a prerequisite for improved post thaw semen quality. Sperm concentration that determines seminal plasma volume in extender regulates quantum of lethal proteins and intensity of lethal interaction in diluted goat semen. Therefore, experiment was designed to evaluate the effect of dilution and sperm concentration in diluted buck semen utilized for cryopreservation and artificial insemination. Semen from four healthy Barbari bucks was collected and pooled. Pooled semen was equally divided and diluted with TRIS based extender containing 6% glycerol and 20% egg yolk at four different sperm concentration viz. 100 million/ml, 200 million/ml, 300 million/ml and 400 million/ml and cryopreserved. Frozen thawed semen was evaluated for progressive motility, live percent, hypo-osmotic swelling test and motility parameters using computer assisted semen analyzer (CASA). A significantly (p < 0.01) higher value for live percent, progressive motility, HOST percent, VCL (μm/sec), VAP (μm/sec), VSL (μm/sec), BCF (hz) and DNC (μm²/sec) were observed in semen diluted to 200-million/ml of sperm concentration. The results depict a better counterbalance between the different factor influencing the viability, membrane integrity, path velocity and motion characters in semen diluted to 200-million/ml of sperms as compared to other sperm concentration in diluted semen. In conclusion, 200-million/ml sperm can be preferred concentration for semen dilution and cryopreservation that result in better post thaw semen quality in buck semen.